Effect of therapeutic administration of beta-lactam antibiotics on the bacterial community and antibiotic resistance patterns in milk

文献类型： 外文期刊

第一作者： Dong, Lei

作者： Dong, Lei;Meng, Lu;Liu, Huimin;Wu, Haoming;Hu, Haiyan;Zheng, Nan;Wang, Jiaqi;Dong, Lei;Meng, Lu;Liu, Huimin;Wu, Haoming;Hu, Haiyan;Zheng, Nan;Wang, Jiaqi;Dong, Lei;Schroyen, Martine

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关键词： dairy cow mastitis; cephalosporin; milk microbiota; antibiotic resistance gene

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2021 年 104 卷 6 期

页码：

收录情况： SCI

摘要： Dairy cows with mastitis are frequently treated with antibiotics. The potential effect of antibiotics on the milk microbiome is still not clear. Therefore, the objective of this research was to investigate the effect of 2 commonly used cephalosporins on the milk microbiota of dairy cows and the antibiotic resistance genes in the milk. The milk samples were collected from 7 dairy cows at the period before medication (d 0), medication (d 1, 2, 3), withdrawal period (d 4, 6, 8), and the period after withdrawal (d 9, 11, 13, 15). We applied 16S rRNA sequencing to explore the microbiota changes, and antibiotic resistance patterns were investigated by quantitative PCR. The microbiota richness and diversity in each sample were calculated using the Chao 1 (richness), Shannon (diversity), and Simpson (diversity) indices. The cephalosporins treatment lowered the Simpson diversity value at the period of withdrawal. Members of the Enterobacter genera were the most affected bacteria associated with mastitis. Meanwhile, antibiotic resistance genes in the milk were also influenced by antibiotic treatment. The cephalosporins treatment raised the proportion of bla(TEM) in milk samples at the period of withdrawal. Therefore, the treatment of cephalosporins led to change in the milk microbiota and increase of beta-lactam resistance gene in the milk at the time of withdrawal period.

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