Citrus exosome-modified exogenous dsRNA delivery reduces plant pathogen resistance and mycotoxin production

文献类型： 外文期刊

第一作者： Yin, Chunxiao

作者： Yin, Chunxiao;Lao, Yuli;Jiang, Yueming;Gong, Liang;Gong, Liang;Xie, Lihong;Chen, Lianfei;Lao, Yuli

作者机构：

关键词： Fungicide resistance; RNAi; Exosomes; dsRNA delivery; Mycotoxin

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2024 年 205 卷

页码：

收录情况： SCI

摘要： Plant-derived exosome-like nanoparticles (PENs) are crucial for intercellular communication. However, PENbased transport of pathogenic fungal genes remains unclear. This study isolated and purified PENs from lane late navel orange citrus juice by following the sucrose gradient ultracentrifugation technique. Citrus PENs were round and oval-shaped with an average size of 154.5 +/- 1.9 nm. Electroporation-based exogenous dsRNA to PENs loading efficiency remained at 6.0 %. Laser confocal microscopy was employed to investigate citrus PEN uptake by fungal spores. dsCrcB loaded PENs inhibited the CrcB gene expression in spores to alleviate Penicillium italicum resistance against prochloraz fungicide, which promoted resistant strains' mortality by 10-fold. Moreover, dsFUM21-loaded PENs suppressed the FUM21 gene expression in spores, which significantly reduced FB1 production in Fusarium proliferatum. These findings suggest that citrus PENs could potentially serve as nano-carriers to counter fungicide resistance and mycotoxin production in pathogenic plant fungi.

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