Accurate Chromosome Identification in the Prunus Subgenus Cerasus (Prunus pseudocerasus) and its Relatives by Oligo-FISH

文献类型： 外文期刊

第一作者： Wang, Lei

作者： Wang, Lei;Feng, Yan;Wang, Yan;Zhang, Jing;Chen, Qing;Liu, Zhenshan;He, Wen;Wang, Hao;Yang, Shaofeng;Zhang, Yong;Luo, Ya;Tang, Haoru;Wang, Xiaorong;Wang, Lei;Feng, Yan;Wang, Yan;Zhang, Jing;Liu, Zhenshan;He, Wen;Wang, Hao;Yang, Shaofeng;Tang, Haoru;Wang, Xiaorong;Liu, Congli

作者机构：

关键词： Prunus (cerasus); chromosome identification; oligonucleotide; FISH; rDNA

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2022 年 23 卷 21 期

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收录情况： SCI

摘要： A precise, rapid and straightforward approach to chromosome identification is fundamental for cytogenetics studies. However, the identification of individual chromosomes was not previously possible for Chinese cherry or other Prunus species due to the small size and similar morphology of their chromosomes. To address this issue, we designed a pool of oligonucleotides distributed across specific pseudochromosome regions of Chinese cherry. This oligonucleotide pool was amplified through multiplex PCR with specific internal primers to produce probes that could recognize specific chromosomes. External primers modified with red and green fluorescence tags could produce unique signal barcoding patterns to identify each chromosome concomitantly. The same oligonucleotide pool could also discriminate all chromosomes in other Prunus species. Additionally, the 5S/45S rDNA probes and the oligo pool were applied in two sequential rounds of fluorescence in situ hybridization (FISH) localized to chromosomes and showed different distribution patterns among Prunus species. At the same time, comparative karyotype analysis revealed high conservation among P. pseudocerasus, P. avium, and P. persica. Together, these findings establish this oligonucleotide pool as the most effective tool for chromosome identification and the analysis of genome organization and evolution in the genus Prunus.

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