Thriving or Withering? Plant Molecular Cytogenetics in the First Quarter of the 21st Century

文献类型： 外文期刊

第一作者： Wolny, Elzbieta

作者： Wolny, Elzbieta;Hasterok, Robert;Mur, Luis A. J.;Ohmido, Nobuko;Yin, Zujun;Yin, Zujun;Wang, Kai

作者机构：

关键词： chromosome; chromosome markers; cytogenetics; cytomolecular analysis; interphase nucleus; FISH; fluorescence in situ hybridisation; karyotype; plant molecular cytogenetics; plant nuclear genome

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 14 期

页码：

收录情况： SCI

摘要： Nearly four decades have passed since fluorescence in situ hybridisation was first applied in plants to support molecular cytogenetic analyses across a wide range of species. Subsequent advances in DNA sequencing, bioinformatic analysis, and microscopy, together with the immunolocalisation of various nuclear components, have provided unprecedented insights into the cytomolecular organisation of the nuclear genome in both model and non-model plants, with crop species being perhaps the most significant. The ready availability of sequenced genomes is now facilitating the application of state-of-the-art cytomolecular techniques across diverse plant species. However, these same advances in genomics also pose a challenge to the future of plant molecular cytogenetics, as DNA sequence analysis is increasingly perceived as offering comparable insights into genome organisation. This perception persists despite the continued relevance of FISH-based approaches for the physical anchoring of genome assemblies to chromosomes. Furthermore, cytogenetic approaches cannot currently rival purely genomic methods in terms of throughput, standardisation, and automation. This review highlights the latest key topics in plant cytomolecular research, with particular emphasis on chromosome identification and karyotype evolution, chromatin and interphase nuclear organisation, chromosome structure, hybridisation and polyploidy, and cytogenetics-assisted crop improvement. In doing so, it underscores the distinctive contributions that cytogenetic techniques continue to offer in genomic research. Additionally, we critically assess future directions and emerging opportunities in the field, including those related to CRISPR/Cas-based live-cell imaging and chromosome engineering, as well as AI-assisted image analysis and karyotyping.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序