文献类型： 外文期刊
第一作者： Wang, Xiao-Bo
作者： Wang, Xiao-Bo;Lu, Hong-Wei;Li, A-Lun;Zhou, Hong-Ling;Ruan, Jue;Wang, Xiao-Bo;Liu, Qing-You;Zhang, Yong;Zhang, Yong;Zhu, Tian-Qi;Zhu, Tian-Qi
作者机构：
关键词： sex-limited chromosome; assembly method; long-read sorting
期刊名称：GIGASCIENCE （ 影响因子：11.8； 五年影响因子：9.4 ）
ISSN： 2047-217X
年卷期： 2024 年 13 卷
页码：
收录情况： SCI
摘要： Background Most currently available reference genomes lack the sequence map of sex-limited (such as Y and W) chromosomes, which results in incomplete assemblies that hinder further research on sex chromosomes. Recent advancements in long-read sequencing and population sequencing have provided the opportunity to assemble sex-limited chromosomes without the traditional complicated experimental efforts.Findings We introduce the first computational method, Sorting long Reads of Y or other sex-limited chromosome (SRY), which achieves improved assembly results compared to flow sorting. Specifically, SRY outperforms in the heterochromatic region and demonstrates comparable performance in other regions. Furthermore, SRY enhances the capabilities of the hybrid assembly software, resulting in improved continuity and accuracy.Conclusions Our method enables true complete genome assembly and facilitates downstream research of sex-limited chromosomes.
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