Wax Crystal-Sparse Leaf1 encodes a beta-ketoacyl CoA synthase involved in biosynthesis of cuticular waxes on rice leaf

文献类型： 外文期刊

第一作者： Yu, Dongmei

作者： Yu, Dongmei;Pei, Zhongyou;He, Chaozu;Yu, Dongmei;Ranathunge, Kosala;Franke, Rochus;Schreiber, Lukas;Huang, Huasun

作者机构：

关键词： cuticular waxes;beta-ketoacyl CoA synthase;rice (Oryza sativa L.);T-DNA insertional mutagenesis;Wax Crystal-Sparse Leaf1;FATTY-ACID ELONGASE;ARABIDOPSIS-THALIANA;EPICUTICULAR WAX;CONDENSING ENZYME;SEQUENCE-ANALYSIS;T-DNA;FUNCTIONAL-CHARACTERIZATION;SUBSTRATE-SPECIFICITY;LESQUERELLA-FENDLERI;EPIDERMAL-CELLS

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

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收录情况： SCI

摘要： Cuticular waxes, forming the plant/atmosphere interface of plants colonizing the terrestrial environment, are complex mixtures of very-long chain fatty acids (VLC-FAs) and their derivatives. In VLCFAs biosynthesis, beta-ketoacyl CoA synthase (E.C.2.3.1.119, KCS) is the key enzyme. Using T-DNA insertional mutagenesis, we identified a cuticle-deficient rice mutant, which displayed a pleiotropic phenotype including reduced growth, leaf fusion, sparse wax crystals, enhanced sensitivity to drought and low fertility. Further analysis indicated that T-DNA was inserted in the 5'-UTR intron of the affected gene, Wax Crystal-Sparse Leaf1 (WSL1), and abnormal transcript caused the loss-of-function of WSL1 gene. Genetic complementation experiment confirmed the function of the candidate gene. WSL1 was predicted to encode a polypeptide containing a conserved FAE1_CUT1_RppA domain typical of the KCS family proteins. Qualitative and quantitative wax composition analyses by gas chromatography-mass spectrometry (GC-MS) demonstrated a marked reduction of total cuticular wax load on wsl1 leaf blades and sheaths, and VLCFA precursors of C20-C24 decreased in both. Moreover, ubiquitous expression of the WSL1 gene gave a hint that WSL1-catalyzed elongation of VLCFAs might participate in a wide range of rice growth and development processes beyond biosynthesis of cuticular waxes.

分类号： Q94

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