The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway

文献类型： 外文期刊

第一作者： Zhang, Wei

作者： Zhang, Wei;Zhang, Minying;Zhang, Keke;Xie, Xuejing;Guo, Chaofan;Shen, Jiyuan;Zhang, Xiaojian;Sun, Huarun;Guo, Liya;Wen, Yuliang;Wang, Lei;Hu, Jianhe;Zhang, Jiaqing;Chen, Sujuan

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关键词： STEAP3; MuSCs myogenic differentiation; iron homeostasis; PI3K/AKT pathway

期刊名称：CELLS （ 影响因子：5.2； 五年影响因子：6.1 ）

ISSN：

年卷期： 2025 年 14 卷 9 期

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收录情况： SCI

摘要： The myogenic differentiation of muscle satellite cells (MuSCs) is an important biological process that plays a key role in the regeneration and repair of skeletal muscles. However, the mechanisms regulating myoblast myogenesis require further investigation. In this study, we found that STEAP3 is involved in myogenic differentiation based on the Yunan black pig MuSCs model in vitro using cell transfection and other methods. Furthermore, the expression of myogenic differentiation marker genes MyoG and MyoD and the number of myotubes formed by the differentiation of cells from the si-STEAP3 treated group were significantly decreased but increased in the STEAP3 overexpression group compared to that in the control group. STEAP3 played a role in iron ion metabolism, affecting myogenic differentiation via the uptake of iron ions and enhancing IRP-IRE homeostasis. STEAP3 also activated the PI3K/AKT pathway, thus promoting myoblast differentiation of Yunan black pig MuSCs. The results of this study showed that STEAP3 overexpression increased intracellular iron ion content and activated the homeostatic IRP-IRE system to regulate intracellular iron ion metabolism.

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