A One-Step Multiplex PCR Method to Rapidly Distinguish Two Strains of Diglyphus wani (Hymenoptera: Eulophidae) Against Agromyzid Leafminers

文献类型： 外文期刊

第一作者： Du, Su-Jie

作者： Du, Su-Jie;Xu, Shi-Yun;Guo, Jian-Yang;Ye, Fu-Yu;Wan, Wei-Jie;Liu, Wan-Xue;Xu, Shi-Yun

作者机构：

关键词： sex determination; morphology; molecular identification; rapid method; mitochondrial gene cytochrome c oxidase I

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0022-0493

年卷期： 2023 年 116 卷 1 期

页码：

收录情况： SCI

摘要： Hymenopteran parasitoids generally show a haplo-diploid sex determination system. Haploid males are produced from unfertilized eggs, whereas diploid females develop from fertilized eggs (arrhenotokous). In some cases, diploid females develop from unfertilized eggs (thelytokous). Diglyphus wani (Hymenoptera: Eulophidae) is a biological control agent for agromyzid leafminers and have arrhenotokous and thelytokous strains. However, the morphological characteristics of two strains of D. wani are so similar that it is difficult to accurately distinguish them based on morphology. Here, a rapid molecular identification method was developed based on the mitochondrial gene cytochrome c oxidase I (COI) and one-step multiplex PCR. Two primer combinations, PC1 (Ar-F1/Th-F1/WR2) and PC2 (Ar-F1/Th-F4/WR2), were designed and repeatedly screened to distinguish two strains simultaneously, of which two special forward primers Th-F1/Th-F4 were used for the thelytokous strain and one special forward primer Ar-F1 was used for the arrhenotokous strain. In addition, a common reverse primer, WR2, was used for both strains. The PC1 and PC2 PCR assays were effective in distinguishing the two strains at different developmental stages and field colonies. This method provides a reliable, highly sensitive, and cost-effective tool for the rapid identification of the two strains of D. wani.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序