Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus

文献类型： 外文期刊

第一作者： Che, Yongliang

作者： Che, Yongliang;Wu, Renjie;Li, Hongjie;Wang, Longbai;Wu, Xuemin;Chen, Qiuyong;Chen, Rujing;Zhou, Lunjiang;Che, Yongliang;Wu, Renjie;Wang, Longbai;Wu, Xuemin;Chen, Qiuyong;Chen, Rujing;Zhou, Lunjiang;Zhou, Lunjiang

作者机构：

关键词： Swine; Glaesserella parasuis; floR; Florfenicol; Small plasmid; Pasteurellaceae family

期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 影响因子：4.6； 五年影响因子：4.1 ）

ISSN： 2213-7165

年卷期： 2023 年 35 卷

页码：

收录情况： SCI

摘要： Objectives: The aim of this study was to characterize the floR-carrying plasmids originating from Glaesserella parasuis and Actinobacillus indolicus isolated from pigs with respiratory disease in China.Methods: A total of 125 G. parasuis and 28 A. indolicus strains collected between 2009 and 2022 were screened for florfenicol resistance. Characterization of floR-positive isolates and plasmids were determined by antimicrobial susceptibility testing, serotyping, multilocus sequence typing (MLST), conjugation and transformation assays, whole-genome sequencing (WGS), and phylogenetic analysis. Results: One A. indolicus and six G. parasuis were identified as positive for floR. The six G. parasuis were assigned to four different serovars, including serovars 6, 7, 9, and unknown. In addition to strain XP11, six floR genes were located on plasmids. The six floR-bearing plasmids could be transformed into Pasteurella multocida and divided into two different types, including similar to 50 0 0 bp and similar to 60 0 0 bp plasmids. The similar to 50 0 0 bp plasmids consisting of rep, lysR, mobB, and floR genes, exhibited high similarity among Pasteurellaceae bacteria. Furthermore, the similar to 60 0 0 bp plasmids, consisting of rep, lysR, mobC, mobA/L, and floR genes, showed high similarity between G. parasuis and Actinobacillus Spp. Notably, WGS results showed that the floR modules of the two types of plasmids could be transferred and integrated into the diverse Pasteurellaceae-origined plasmids.Conclusion: This study firstly reported the characterization of floR-carrying plasmids from A. indolicus and a non-virulent serovar of G. parasuis in pigs in China and elucidated the transmission mechanism of the floR resistance gene among the Pasteurellaceae family.(c) 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )

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