Fine mapping of qDB.A03, a QTL for rapeseed branching, and identification of the candidate gene

文献类型： 外文期刊

第一作者： Li, Bao

作者： Li, Bao;Wang, Tonghua;Guo, Yiming;Liu, Xinhong;Deng, Lichao;Qu, Liang;Li, Mei;Li, Bao;Wang, Tonghua;Guo, Yiming;Liu, Xinhong;Deng, Lichao;Qu, Liang;Li, Mei

作者机构：

关键词： Brassica napus; Gene mapping; Dense branching; Shoot apical meristems

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：2.98； 五年影响因子：3.207 ）

ISSN： 1617-4615

年卷期： 2022 年 297 卷 3 期

页码：

收录情况： SCI

摘要： Increasing the yield of rapeseed is required to meet the rapidly expanding demand for both edible vegetable oil and biofuel. Branching, an important determinant of yield potential in rapeseed, is controlled by a series of quantitative trait loci (QTLs). To explore the genetic mechanism regulating the natural variation of branching, a BC1F1 population derived from a cross between dense branching 2 (dense branching line) and L72 (normal branching line) was used to map QTL conferring branching in rapeseed. A major QTL, qDB.A03, for branching-related traits was identified by the BeadChip Array assisted bulked segregation analysis method, which was subsequently validated by the classical QTL mapping approach, and fine mapped to the 256 kb region. This interval contains 56 annotated or predicted genes, 8 of which are candidates for controlling the branching trait. Comparative and expression analysis revealed four promising candidate genes for qDB.A03. Fine mapping and identification of the candidate genes for qDB.A03 represents the first step toward unraveling the genetical and molecular mechanisms controlling branching in rapeseed.

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