Comprehensive profiling and evaluation of free/conjugated Phytosterols crops using chemical derivatization coupled with UHPLC-ESI-QTOF-MS/ MS

文献类型： 外文期刊

第一作者： Xu, Qiuhui

作者： Xu, Qiuhui;Wang, Dan;Lv, Xin;Chen, Hong;Wei, Fang;Wei, Fang

作者机构：

关键词： Detection sensitivity; Distinctive marker; DNSCl derivatization; Phytosterol profile; Precursor ion scanning

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 463 卷

页码：

收录情况： SCI

摘要： Phytosterols are naturally existed in crops but their detection is constrained by sensitivity and accuracy due the inefficient analytical approaches. This study hypothesizes that an untargeted analytical method combining chemical derivatization with ultrahigh performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry can identify the various composition and contents of phytosterols in different crops. The results showed that chemical derivatization significantly enhanced intensity of phytosterols compared with non-derivatized samples. Using precursor ion scanning (PIS) of m / z 252.0690, dansyl chloride-labeled phytosterols were identified, demonstrating that rapeseeds had the highest content of total phytosterol (3981.2 +/- 95.3 mg/kg), followed by sunflower seeds, flaxseeds, corn and rice, respectively. Principal component analysis revealed significant variations in phytosterol distribution among 15 crop samples, suggesting applicability of phytosterol profile as a marker for phytosterols-contained crops. Hence, the proposed analytic approach proves high efficiency and accuracy in determining phytosterols and advances the study phytosterol-enriched crops.

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