Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption

文献类型： 外文期刊

第一作者： Zhang, Yi

作者： Zhang, Yi;He, Yongding;Zhang, Shilong;Zhou, Sisi;Shen, Wankuan;Zhang, Yi;He, Yongding;Zhang, Shilong;Zhou, Sisi;Shen, Wankuan;Yan, Meixin

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关键词： sugarcane; Sporisorium scitamineum; Major Facilitator Superfamily (MFS) sugar transporter; sexual mating; pathogenicity

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.8； 五年影响因子：4.1 ）

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年卷期： 2025 年 13 卷 2 期

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收录情况： SCI

摘要： Sugarcane smut caused by Sporisorium scitamineum is a global sugarcane disease, and studying its molecular pathogenesis is crucial for discovering new prevention and control targets. This study was based on the transcriptome sequencing data of two isolates with different pathogenicities (Ss16 and Ss47) of the S. scitamineum and screened out a gene encoding the Major Facility Superfamily (MFS) sugar transporter protein and named it SsMFSST1. Knockout mutants (triangle SsMFSST1(+) and triangle SsMFSST1(-)) and complementary mutants (COMMFSST1(+) and COMMFSST1(-)) were obtained through polyethylene glycol (PEG)-mediated protoplast transformation technology. On this basis, the function of gene SsMFSST1 was analyzed. The research results showed that the sexual mating ability of the knockout mutants significantly decreased compared with the wild type, while the sexual mating ability of the complementary mutants was almost restored to the level of the wild type. After the addition of exogenous small molecular signaling substance cyclic adenosine monophosphate (cAMP) or tryptophol required for the sexual mating of S. scitamineum, the sexual mating ability of the knockout mutants was almost restored to the level of the wild type. It was observed that the expression levels of the key genes Uac1 for cAMP synthesis and Aro8 for tryptophol synthesis were significantly lower in the knockout mutants compared with the wild type. However, the expression levels of these genes in the complementary mutants were restored to the wild-type levels. The pathogenicity testing also found a significant decrease in the pathogenicity of combinations containing mutants. On YePSA medium, the gene SsMFSST1 does not affect the growth, spore morphology, colony morphology, and oxidative stress ability of the haploid spores of S. scitamineum. Therefore, we speculate that the SsMFSST1 gene may regulate the expression of key genes related to the synthesis pathway of the small molecule signaling substance cAMP or tryptophol, affecting the synthesis of the signaling substance cAMP or tryptophol, thereby affecting the sexual mating and pathogenicity of S.scitamineum. In addition, this gene also regulates the transport/absorption of fructose and lactose in S. scitamineum. In summary, this study identified a novel pathogenic gene in the S. scitamineum-the MFS sugar transporter gene, providing a molecular basis for understanding the pathogenic mechanism of the S. scitamineum. On the other hand, it also enriches the biological functions of this gene in fungi.

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