Single-cell transcriptome landscape of zebrafish liver reveals hepatocytes and immune cell interactions in understanding nonalcoholic fatty liver disease

文献类型： 外文期刊

第一作者： Huang, Yingyi

作者： Huang, Yingyi;Liu, Xiang;Wang, Hong-Yan;Li, Yubang;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Huang, Yingyi;Liu, Xiang;Wang, Hong-Yan;Li, Yubang;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Chen, Jian-Yang;Zhang, Xianghui;Lu, Yifang;Wang, Qian;Fan, Guangyi;Chen, Jian-Yang;Zhang, Xianghui;Lu, Yifang;Fan, Guangyi;Dong, Zhongdian;Wang, Zhongduo;Liu, Shanshan;Fan, Guangyi;Liu, Shanshan;Zou, Jun

作者机构：

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 146 卷

页码：

收录情况： SCI

摘要： Nonalcoholic fatty liver disease (NAFLD) is becoming the most common chronic liver disease in the world. Immunity is the major contributing factor in NAFLD; however, the interaction of immune cells and hepatocytes in disease progression has not been fully elucidated. As a popular species for studying NAFLD, zebrafish, whose liver is a complex immune system mediated by immune cells and non -immune cells in maintaining immune tolerance and homeostasis. Understanding the cellular composition and immune environment of zebrafish liver is of great significance for its application in NAFLD. Here, we established a liver atlas that consists of 10 cell types using single -cell RNA sequencing (scRNA-seq). By examining the heterogeneity of hepatocytes and analyzing the expression of NAFLD-associated genes in the specific cluster, we provide a potential target cell model to study NAFLD. Additionally, our analysis identified two subtypes of distinct resident macrophages with inflammatory and non -inflammatory functions and characterized the successive stepwise development of T cell subclusters in the liver. Importantly, we uncovered the possible regulation of macrophages and T cells on target cells of fatty liver by analyzing the cellular interaction between hepatocytes and immune cells. Our data provide valuable information for an in-depth study of immune cells targeting hepatocytes to regulate the immune balance in NAFLD.

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