Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens

文献类型： 外文期刊

第一作者： Lu, Huipeng

作者： Lu, Huipeng;Zhou, Xiaohui;Zhang, Xinyu;Zhu, Liqi;Zhang, Quan;Sun, Huaichang;Wu, Zhi;Zhu, Shanyuan;Sun, Huaichang;Guo, Xiaoyu;Zhu, Hongfei

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关键词： Recombinant adenovirus vector; African swine fever virus antigens; Toll-like receptor ligands; Mucosal adjuvanticity

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

ISSN： 0165-2427

年卷期： 2021 年 239 卷

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收录情况： SCI

摘要： The mucosal immunity plays an important role against African swine fever virus (ASFV) infection and the ef-ficacy of mucosal vaccination is highly dependent on the adjuvant. However, the mucosal adjuvant for ASFV vaccination is poorly studied. Toll-like receptor (TLR) ligands such as the FlaB flagellin from Vibrio vulnificus and the heat shock protein 70 from Mycobacterium tuberculosis (mHsp70) hold a great promise as novel vaccine adjuvant. However, the mucosal adjuvanticities of such TLR ligands have not been studied in pigs. In this study, three recombinant Adenovirus (rAd) vectors, namely rAd-F1, rAd-FlaB-F1 and rAd-F1-Hsp70, were constructed by fusing the FlaB or mHsp70 to ASFV CD2v-p30-p54 fusion antigen. Western blotting showed that the three fusion proteins expressed in rAd-infected cells reacted positively with ASFV antibodies. After intranasal immu-nization of pigs with the three rAd vectors, the antigen-specific IgG antibodies were detectable from day 7 after primary immunization, which were significantly boosted by the secondary immunization. Strong Th1/Th2 cytokine responses were detected in the peripheral blood mononuclear cells. Compared to immunization with the control rAd-F1, significantly higher levels of the antigen-specific IgA antibodies were detected in the nasal fluids, tracheal washes and lung lavages.1 Compared to immunization with rAd-Flab-F1, immunization with rAd-F1-Hsp70 induced significantly stronger mucosal IgA antibody response. Cytokine detection of the pig lung la-vages showed that the elevated IgA antibody responses were correlated mainly with IL-4, IL-10 and IFN-alpha, which were confirmed by the significantly increased antigen-recall cytokine expression in the porcine alveolar mac-rophages. These data suggest that mHsp70 has potent mucosal adjuvanticity in pigs, and the fusion rAd vector can be used for ASFV mucosal vaccine development.

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