Biological characterization of the melanin biosynthesis gene Bcscd1 in the plant pathogenic fungus Botrytis cinerea

文献类型： 外文期刊

第一作者： Zhou, Yingjun

作者： Zhou, Yingjun;Song, Juanjuan;Wang, Yongchun;Yang, Long;Wu, Mingde;Li, Guoqing;Zhang, Jing;Zhou, Yingjun;Song, Juanjuan;Wang, Yongchun;Yang, Long;Wu, Mingde;Li, Guoqing;Zhang, Jing;Zhou, Yingjun

作者机构：

关键词： Botrytis cinerea; Melanin; Bcscd1; Cell wall integrity; Biotic/abiotic stresses

期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.883； 五年影响因子：3.931 ）

ISSN： 1087-1845

年卷期： 2022 年 160 卷

页码：

收录情况： SCI

摘要： The gray mold fungus Botrytis cinerea produces dark-colored conidia and sclerotia due to deposition of melanin on the cell wall of these structures. However, the role of melanin biosynthesis on development and function of conidia and sclerotia have not been well elucidated in this fungus. This study disrupted the melanin biosynthesis gene Bcscd1 (for scytalone dehydratase) in the wild type B05.10, and the resulting mutants were compared with B05.10 and complementary mutants (COM) for growth and development, virulence and response to biotic/abiotic stresses. Three disruption mutants were obtained, and they did not differ from B05.10 and COM in mycelial growth rate on potato dextrose agar, however, they formed brownish conidia and scleotia deficient in melanogenesis, whereas B05.10 and COM formed grayish conidia and black sclerotia with normal melanogenesis. The disruption mutants were as aggressive as B05.10 and COM in infection of tobacco leaves. TEM observation showed that the disruption mutant delta Scd1-85 formed numerous tiny grooves in the conidial cell wall, thereby causing uneven thickness in the cell wall. In contrast, B05.10 and COM rarely formed tiny grooves in their conidial cell wall with even thickness. Moreover, the sclerotial cortex cell wall of delta Scd1-85 lost rigidity and the cells became collapsed, whereas the sclerotial cortex cell wall of B05.10 and COM appeared rigid, and the cells appeared plump in shape. The disruption mutants were more sensitive than B05.10 and COM in response to chemical stresses (H2O2, NaCl, SDS, sorbitol) for conidial germination and sclerotial survival. The sclerotia of the disruption mutants were more susceptible than the sclerotia of B05.10 and COM to infection by the mycoparasite Trichoderma koningiopsis. These results confirmed previous studies about the effect of melanin production on pathogenicity of B. cinerea, and expanded our knowledge about the role of Bcscd1 in cell wall integrity and in response to biotic and abiotic stresses.

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