Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'

文献类型： 外文期刊

第一作者： Xi, Yinkai

作者： Xi, Yinkai;Huang, Hengyu;Wang, Ye;Yang, Pinhui;Xi, Yinkai;Zeng, Biao;Huang, Hengyu;Wang, Ye

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关键词： sweet cherry 'Fuchen'; bud proliferation; basal medium; rapid propagation; browning inhibition

期刊名称：HORTICULTURAL SCIENCE （ 影响因子：1.192； 五年影响因子：1.273 ）

ISSN： 0862-867X

年卷期： 2022 年 49 卷 1 期

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收录情况： SCI

摘要： Prunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomenon. The results indicated that 1.0 g/L of AC and 30 g/L of Na(2)S(2)O(3)were the best combination to inhibit the browning on the optimal basal medium - olive medium (OM) (followed by woody plant medium). The OM containing 1.5 mg/L of zeatin, 2.0 mg/L of alpha-naphtalenaecetic acid (NAA) and 0.5 mg/L of kinetin was beneficial for the germination and growth of the axillary buds. Green bud points were visible at the base of the swollen base after 25 days with a high proliferation coefficient (more than 7.50). The most optimal medium for rooting was 1/2 OM combined with 1.0 mg/L of NAA and 0.5 mg/L of indole-3-butyric-acid with a 100% rooting rate. The survival rate was up to 100% after 60 days with acclimatisation. Generally, a high-effective regeneration system was established, which provides a reference for keeping the excellent traits of the cultivar.

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