Comparative Genomics of Three Novel Jumbo Bacteriophages Infecting Staphylococcus aureus

文献类型： 外文期刊

第一作者： Korn, Abby M.

作者： Korn, Abby M.;Gill, Jason J.;Korn, Abby M.;Sun, Lichang;Gill, Jason J.;Hillhouse, Andrew E.;Hillhouse, Andrew E.;Sun, Lichang

作者机构：

关键词： Staphylococcus aureus; bacteriophage; jumbo phage; MarsHill; Madawaska; Machias

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）

ISSN： 0022-538X

年卷期： 2021 年 95 卷 19 期

页码：

收录情况： SCI

摘要： The majority of previously described Staphylococcus aureus bacteriophages belong to three major groups, namely, P68-like podophages, Twort-like or K-like myo-phages, and a more diverse group of temperate siphophages. Here, we present the fol-lowing three novel S. aureus "jumbo" phages: MarsHill, Madawaska, and Machias. These phages were isolated from swine production environments in the United States and represent a novel clade of S. aureus myophage. The average genome size for these phages is similar to 269 kb with each genome encoding similar to 263 predicted protein-coding genes. Phage genome organization and content are similar to those of known jumbo phages of Bacillus sp., including AR9 and vB_BpuM-BpSp. All three phages possess genes encod-ing complete virion and nonvirion RNA polymerases, multiple homing endonucleases, and a retron-like reverse transcriptase. Like AR9, all of these phages are presumed to have uracil-substituted DNA which interferes with DNA sequencing. These phages are also able to transduce host plasmids, which is significant as these phages were found circulating in swine production environments and can also infect human S. aureus isolates. IMPORTANCE This study describes the comparative genomics of the following three novel S. aureus jumbo phages: MarsHill, Madawaska, and Machias. These three S. aureus myophages represent an emerging class of S. aureus phage. These genomes contain abundant introns which show a pattern consistent with repeated acquisition rather than vertical inheritance, suggesting intron acquisition and loss are active processes in the evolution of these phages. These phages have presumably hypermodified DNA which inhibits sequencing by several different common platforms. Therefore, these phages also represent potential genomic diversity that has been missed due to the limitations of standard sequencing techniques. In particular, such hypermodified genomes may be missed by metagenomic studies due to their resistance to standard sequencing techni-ques. Phage MarsHill was found to be able to transduce host DNA at levels comparable to that found for other transducing S. aureus phages, making it a potential vector for horizontal gene transfer in the environment.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序