A novel linear epitope at the C-terminal region of the classical swine fever virus E2 protein elicits neutralizing activity

文献类型： 外文期刊

第一作者： Xu, Qianru

作者： Xu, Qianru;Zhang, Gaiping;Xu, Qianru;Liu, Linke;Zhang, Shenli;Niu, Xiangxiang;Li, Xueyang;Zhang, Erqin;Zhang, Gaiping;Xu, Qianru;Liu, Linke;Zhang, Shenli;Niu, Xiangxiang;Li, Xueyang;Zhang, Erqin;Zhang, Gaiping;Xu, Qianru;Guo, Junqing;Li Wang;Liu, Yunchao;Li, Qingmei;Chai, Shujun;Zhang, Gaiping;Wang, Yanan;Jiang, Min;Wang, Yanwei;Wang, Ruining;Ma, Qiang;Ma, Fanshu;Zhang, Gaiping

作者机构：

关键词： Classical swine fever virus; Neutralizing monoclonal antibodies; Linear B-cell epitope

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 189 卷

页码：

收录情况： SCI

摘要： Classical swine fever virus (CSFV) is a member of the genus Pestivirus, which causes serious economic losses. The re-emergence of the disease in Japan in 2018 has increased awareness of CSFV. In this study, Balb/c mice were immunized with plant-derived E2 protein, and four monoclonal antibodies (mAbs) 4B11, 7B3, 11A5 and 6F3 were generated. Two of these mAbs, 4B11 and 7B3, effectively blocked CSFV infection of PK-15 cells. Both mAbs recognized a novel linear epitope, (256)CLIGNTTVKVHASDER(271). The neutralizing ability of anti-CSFV serum decreased 63%, when pre-incubated with the linear peptide at 200 mu g/mL. Structural analysis showed that this linear epitope is present at the border of Domain C and Domain D on the surface of the E2 protein. Alignment of amino acid sequences showed that the epitope was conserved in different subgroups of CSFV but not in other members of the Pestivirus genus. Consistently with the analysis above, this epitope distinguished antibodies against CSFV from those against bovine viral diarrhea virus (BVDV). Our study provides an ideal candidate peptide for new vaccine design and differential diagnosis of CSFV. These findings will contribute to the control and eradication of classical swine fever.

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