Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV

文献类型： 外文期刊

第一作者： Yan Fei

作者： Yan Fei;Zheng Yinying;Zhang Wenwei;Xiao Hong;Li Shifang;Cheng Zhuomin

作者机构：

关键词： pad;Agrobacterium -mediated transformation;wheat;transgenic;antivirus;Barley yellow dwarf virus

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： In fission yeast (Schizosaccharomyces pombe), pac1 gene was cloned with 99.3 percent nucleo-tide sequence similarity with published pad in GenBank. In pET-5cs expression system, the expression product of cloned pac1 in E. coll showed activity to degrade the double-strand RNA. Harboring the binary vector pBI121, which contains pad gene, Agrobacterium tumefaciens strain LBA4404 was used to transform the wheat immature embryos pre-cultured 7-10 d. After preregeneration, regeneration and selection culture stage, totally 41 G418 resistant plants were obtained, in which 25 lines were proved to integrate with transgene and express transgene normally by PCR, Dot blot, RT-PCR and ELISA detection. Antivirus test carried out on 25 positive lines with high dose of Barley yellow dwarf virus-GPV revealed that 12 lines had resistance to BVDV-GPV in low level, another 12 lines had resistance to BVDV-GPV in middle level, and 1 line showed resistance to BVDV-GPV in high level. However, both low and middle level of resistance plants showed no symptoms when infected by viruses at low dose, which suggested the dose-dependent effect of the resistance mediated by pad to BYDV-GPV.

分类号： N1

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