Novel exopolysaccharide from Limosilactobacillus fermentum A10: Biosynthesis, physicochemical properties and antioxidant activity

文献类型： 外文期刊

第一作者： Wei, Guangqiang

作者： Wei, Guangqiang;Zhang, Wenbin;Li, Yufang;Wang, Daodian;Chai, Yunmei;Wang, Yuzhu;Xu, Ziqi;Hu, Shaomei;Shi, Jindou;Huang, Aixiang;Zhao, Xingwen;Wang, Daodian

作者机构：

关键词： Exopolysaccharide; Physicochemical properties; Antioxidant activity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 312 卷

页码：

收录情况： SCI

摘要： In this study, the biosynthesis, physicochemical properties and antioxidant activity of novel EPS from Limosilactobacillus fermentum A10 were investigated. Whole genome sequencing and functional annotation were employed to identify genes involved in the EPS synthesis and the Wzx/Wzy-dependent pathway. After being purified by a DEAE-52 cellulose column, the main component EPS-1, which consisted of rhamnose, galactose, glucose, mannose, and glucuronic acid, was isolated and prepared. Structural analysis indicated that EPS-1 was a heteropolysaccharide containing a pyranose ring with a zeta potential of -18 mV and a particle size of 146 nm. EPS-1 had a loose, porous, crystalline structure and had thermal stability of up to 257.80 degrees C. EPS-1 exhibited high radical scavenging activity and cytoprotective effect against H2O2-induced HepG2 cellular oxidative damage and significantly increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT). Moreover, EPS-1 induced antioxidant mechanisms by activating the Keap1-Nrf2/ARE signaling pathway. Molecular docking results indicated that the main interactions between Keap1 protein and rhamnose, galactose, glucose, mannose, and glucuronic acid are hydrogen bonds (N-H and O-H) and electrostatic interactions (amide bonds). These results demonstrated that EPS-1 can potentially be used in functional food to target Keap1 inhibitors/Nrf2 activators.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序