Production of N-acetylglucosamine from carbon dioxide by engineering Cupriavidus necator H16

文献类型： 外文期刊

第一作者： Wang, Xiaolu

作者： Wang, Xiaolu;Chang, Fangfang;Wang, Tingting;Luo, Huiying;Su, Xiaoyun;Tu, Tao;Wang, Yuan;Bai, Yingguo;Qin, Xing;Zhang, Honglian;Wang, Yaru;Yao, Bin;Huang, Huoqing;Zhang, Jie

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关键词： Metabolic engineering; N-acetylglucosamine; CO2; Cupriavidus necator

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 379 卷

页码：

收录情况： SCI

摘要： The conversion of CO2 into valuable bioactive substances using synthetic biological techniques is a potential approach for mitigating the greenhouse effect. Here, the engineering of C. necator H16 to produce N-acetyl-glucosamine (GlcNAc) from CO2 is reported. First, GlcNAc importation and intracellular metabolic pathways were disrupted by the deletion of nagF, nagE, nagC, nagA and nagB genes. Second, the GlcNAc-6-phosphate N- acetyltransferase gene (gna1) was screened. A GlcNAc-producing strain was constructed by overexpressing a mutant gna1 from Caenorhabditis elegans. A further increase in GlcNAc production was achieved by disrupting poly(3-hydroxybutyrate) biosynthesis and the Entner-Doudoroff pathways. The maximum GlcNAc titers were 199.9 and 566.3 mg/L for fructose and glycerol, respectively. Finally, the best strain achieved a GlcNAc titer of 75.3 mg/L in autotrophic fermentation. This study demonstrated a conversion of CO2 to GlcNAc, thereby providing a feasible approach for the biosynthesis of various bioactive chemicals from CO2 under normal conditions..

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