Genome-wide Identification fi cation and Functional Analyses of the 5-O-Glucosyltransferase Family Reveal Its Potential Roles in Anthocyanidin Diglucosides Synthesis of Grape Skins with Vitis amurensis Pedigree

文献类型： 外文期刊

第一作者： Zhu, Lei

作者： Zhu, Lei;Chen, Yunhua;Liu, Yunqing;Li, Dandan;Wang, Yuanyuan;Liu, Yunting;Xu, Jingyu;Zhu, Lei;Zhu, Lei;Yang, Yiming;Wang, Mingjie;Hu, Xixi;Hu, Xixi

作者机构：

关键词： 5GT family; allele; anthocyanidin diglucosides; gene expression; Vitis amurensis

期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.2； 五年影响因子：1.6 ）

ISSN： 0003-1062

年卷期： 2024 年 149 卷 4 期

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收录情况： SCI

摘要： A BSTRACT . Vitis amurensis grape cultivars and hybrids are mainly used to make wines in Northeast Asia with a cold winter. Anthocyanidin glucosylation at 5-O position catalyzed by 5-O-glucosyltransferase (5GT) in grape skins is crucial for color stability of red wines. To study 5GT functions in anthocyanidin diglucosides synthesis of V. amurensis, , 20 5GTs s were preliminarily identified fi ed from a genome-wide characterization of the UDP-glycosyltransferase family according to the reported 5GT s, which were also performed phylogenetic and bioinformatics analysis. Two important 5GT s, Vitvi0900582.t01 and Vitvi05g01269.t01, , were screened through analyses of anthocyanidin diglucosides accumulation and gene expression in berry skins of three representative grape cultivars with significant fi cant differences in anthocyanin glycosylation. Fourteen alleles of each of the 5GTs s were cloned from 14 V. amurensis cultivars and hybrids as well as from V. vinifera ' Cabernet Sauvignon', ' , and sequence analysis and functional prediction were performed. From three perspectives of phenotype, transcriptional level, and genotype, it has been found that the functional allele at the Vitvi0900582.t01 locus of Chr 9 played a decisive role in the synthesis of abundant anthocyanidin diglucosides in V. amurensis. . In addition, the trace anthocyanidin diglucosides detected in V. vinifera ' Cabernet Sauvignon' ' were led by the functional allele at the Vitvi05g01269.t01 locus of Chr 5. This study provides preliminary data for further research on the regulatory mechanism of anthocyanidin diglucosides in the grapes with the V. amurensis pedigree to improve their wine quality in future breeding efforts.

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