PPAR gamma regulates fabp4 expression to increase DHA content in golden pompano (Trachinotus ovatus) hepatocytes

文献类型： 外文期刊

第一作者： Lei, Caixia

作者： Lei, Caixia;Fan, Bin;Li, Mengmeng;Li, Yuanyou;Lei, Caixia;Fan, Bin;Li, Mengmeng;Li, Yuanyou;Lei, Caixia;Tian, Jingjing;Fan, Bin;Li, Mengmeng

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关键词： fatty acid-binding protein 4; n-3 long-chain PUFA; Uptake and deposition; Trachinotus ovatus

期刊名称：BRITISH JOURNAL OF NUTRITION （ 影响因子：4.125； 五年影响因子：4.862 ）

ISSN： 0007-1145

年卷期： 2022 年 127 卷 1 期

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收录情况： SCI

摘要： N-3 long-chain (>= C20) PUFA (LC-PUFA) are vital fatty acids for fish and humans. As a main source of n-3 LC-PUFA for human consumers, the n-3 LC-PUFA content of farmed fish is important. Previously, we identified fatty acid-binding protein (fabp)-4 as a candidate gene for regulating the n-3 LC-PUFA content. Herein, we further assessed the role of fabp4 in this process. First, a 2059 bp promoter sequence of fabp4 in Trachinotus ovatus was cloned and, using progressive deletion, determined -2006 bp to -1521 bp to be the core promoter sequence. The PPAR-gamma binding sites were predicted to occur in this region. A luciferase reporter assay showed that the promoter activity of fabp4 decreased following mutation of the PPAR gamma binding site and that PPAR gamma increased the fabp4 promoter activity in a dose-dependent manner, implying that T. ovatus fabp4 is a target of PPAR gamma. The overexpression of fabp4 or PPAR gamma increased the DHA content in hepatocytes, whereas suppression of their expression diminished this effect, suggesting that both fabp4 and PPAR gamma play an active role in regulating DHA content. Moreover, the inhibition of fabp4 attenuated the increase in PPAR gamma-mediated DHA content, and the overexpression of fabp4 alleviated this effect. Collectively, our findings indicated that fabp4, which is controlled by PPAR gamma, plays an important role in DHA content regulation. The new regulation axis can be considered a promising novel target for increasing the n-3 LC-PUFA content in T. ovatus.

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