TAGGING PATHOGENICITY GENES IN FUSARIUM GRAMINEARUM USING THE TRANSPOSON SYSTEM MIMP/IMPALA

文献类型： 外文期刊

第一作者： Ben M'barek, Sarrah

作者： Ben M'barek, Sarrah;Liu, Taiguo;Daboussi, Marie-Josee;Van der Lee, Theo;Ben M'barek, Sarrah;Xu, X.;Zhang, X.;Zhang, Wenwei;Kema, Gert H. J.;Waalwijk, Cees;Xu, X.;Zhang, X.;Liu, Taiguo;Zhang, Wenwei

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期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：0.85； 五年影响因子：1.067 ）

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收录情况： SCI

摘要： Transposon mutagenesis was applied to generate mutants in Fusarium graminearum. The mimpl/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) ofgenes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation witha non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.

分类号： S5

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