Integrated Transcriptome and Metabolome Analysis Reveals Mechanism of Flavonoid Synthesis During Low-Temperature Storage of Sweet Corn Kernels

文献类型： 外文期刊

第一作者： Xiao, Yingni

作者： Xiao, Yingni;Hu, Jianguang;Li, Gaoke;Liu, Jingyan;Zhao, Xu;Du, Jin;Jin, Weiwei

作者机构：

关键词： sweet corn; low-temperature storage; transcriptome; integrated analysis; flavonoid synthesis

期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）

ISSN：

年卷期： 2024 年 13 卷 24 期

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收录情况： SCI

摘要： Sweet corn is a globally important food source and vegetable renowned for its rich nutritional content. However, post-harvest quality deterioration remains a significant challenge due to sweet corn's high sensitivity to environmental factors. Currently, low-temperature storage is the primary method for preserving sweet corn; however, the molecular mechanisms involved in this process remain unclear. In this study, kernels stored at different temperatures (28 degrees C and 4 degrees C) for 1, 3, and 5 days after harvest were collected for physiological and transcriptomic analysis. Low temperature storage significantly improved the PPO and SOD activity in sweet corn kernels compared to storage at a normal temperature. A total of 1993 common differentially expressed genes (DEGs) were identified in kernels stored at low temperatures across all three time points. Integrated analysis of transcriptomic and previous metabolomic data revealed that low temperature storage significantly affected flavonoid biosynthesis. Furthermore, 11 genes involved in flavonoid biosynthesis exhibited differential expression across the three storage periods, including CHI, HCT, ANS, F3 ' H, F3 ' 5 ' H, FLS, and NOMT, with Eriodictyol, Myricetin, and Hesperetin-7-O-glucoside among the key flavonoids. Correlation analysis revealed three AP2/ERF-ERF transcription factors (EREB14, EREB182, and EREB200) as potential regulators of flavonoid biosynthesis during low temperature treatment. These results enhance our understanding of the mechanisms of flavonoid synthesis in sweet corn kernels during low-temperature storage.

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