Enhanced mycoprotein production of Neurospora intermedia from soy processing byproducts: Integration of ultrasonic stimulation with repeated-batch simultaneous saccharification and culture

文献类型： 外文期刊

第一作者： Dai, Yiqiang

作者： Dai, Yiqiang;Wang, Zhe;Han, Siyu;Wang, Daoying;Xia, Xiudong;Dai, Yiqiang;Liu, Yifei;Tao, Yang;Dong, Mingsheng;Wang, Daoying;Xia, Xiudong;Wang, Zhongjiang

作者机构：

关键词： Neurospora intermedia mycoprotein; Soy whey; Okara; Ultrasound treatment; Repeated-batch culture

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.0； 五年影响因子：9.5 ）

ISSN： 0960-8524

年卷期： 2025 年 437 卷

页码：

收录情况： SCI

摘要： This study developed an integrated strategy combining simultaneous saccharification and culture (SSC), lowfrequency ultrasound treatment with repeated-batch culture (RBC) for Neurospora intermedia mycoprotein production from soy whey and okara. Results showed that soy whey served as a desired substrate for mycoprotein production, and adding okara increased both mycoprotein yield and productivity by balancing the carbon-tonitrogen ratio. Ultrasound treatment further increased the mycoprotein yield and shortened the production time by facilitating material exchange and improving cellulase activity. Finally, this integrated strategy was applied in shake-flask and 5 L fermenter systems, and their mycoprotein productivities of 1.79 +/- 0.03 and 2.11 +/- 0.04 g/L/12 h were achieved, showing 98.72 % and 189.04 % increases compared with those in soy whey alone with batch culture (BC). Moreover, the chemical and biological oxygen demand removal ratios reached 73.41 +/- 0.69 % and 94.38 +/- 0.78 %. Overall, this study offers an efficient, economical and environmentally sustainable way for mycoprotein production from agriculture and food industry waste.

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