Map-based cloning and characterization of yg-2, a gene conferring yellow-green leaf in tomato (Solanum lycopersicum)

文献类型： 外文期刊

第一作者： Su, Xiaomei

作者： Su, Xiaomei;Lyu, Hongjun;Li, Jing;Liu, Shumei;Hou, Lixia;Gao, Jianchang

作者机构：

关键词： Tomato; Yellow-green leaf; yg-2; Gene clone; Chlorophyll

期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）

ISSN： 1380-3743

年卷期： 2024 年 44 卷 12 期

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收录情况： SCI

摘要： Leaves play a critical role in plant growth and development, directly influencing crop yield through their essential functions in photosynthesis and respiration. This study employed inheritance analysis and gene mapping of an F2 population derived from a cross between a spontaneous yellow-green leaf tomato mutant and a wild-type tomato line. The findings conclusively demonstrated that the yellow-green leaf trait is controlled by a single recessive gene. Subsequent fine-mapping localized this gene to a 270-kb region on chromosome 12 of the reference Heinz 1706 genome. Annotation and functional characterization of genes within this region indicated Solyc12g009470 (yg-2) as the primary candidate gene influencing the yellow-green phenotype trait. Sequencing analysis revealed a 49-bp deletion in the first exon of yg-2, resulting in suppressed yg-2 expression. This functional role was further confirmed through Solyc12g009470 gene editing in tomatoes. Moreover, comparative analyses of photosynthetic pigments and chloroplast ultrastructure revealed notable differences between the mutant and the wild-type lines. Furthermore, the mutant exhibited reduced photosynthetic rate and yield-related agronomic traits. These findings provide valuable insights into the molecular mechanisms underlying yellow-green leaf formation in tomatoes.

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