The tetratricopeptide repeat protein TaTPR-B1 regulates spike compactness in bread wheat

文献类型： 外文期刊

第一作者： Zhu, Jun

作者： Zhu, Jun;Huang, Feng;Zhai, Huijie;Zheng, Yi;Yu, Jiazheng;Chen, Zhaoyan;Fan, Yujie;Zhao, Huanhuan;Sun, Qixin;Liang, Rongqi;Ni, Zhongfu;Huang, Feng;Zhai, Huijie;Chen, Zhaoyan

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.9； 五年影响因子：7.7 ）

ISSN： 0032-0889

年卷期： 2024 年 197 卷 1 期

页码：

收录情况： SCI

摘要： Spike compactness (SC) is strongly associated with wheat (Triticum aestivum L.) grain yield. In this study, we conducted a quantitative trait locus (QTL) analysis using a doubled haploid (DH) population derived from a cross between 2 common wheat varieties with contrasting spike morphology, revealing 16 stable QTLs associated with SC. The effect of a major QTL, QSc.cau-6B.1, was validated in 231 F7 recombinant inbred lines (RILs) derived from the same cross as the DH population. Using 2 residual heterozygous lines (RHLs), we delimited QSc.cau-6B.1 to a similar to 0.5-Mbp physical interval containing four high-confidence genes. The tetratricopeptide repeat-TraesCS6B03G1214400 (TaTPR-B1) was the priority candidate gene according to sequence and expression variations between near-isogenic lines. Accordingly, TaTPR-B1 knockout in the common wheat variety "CB037" significantly increased SC compared with the wild type (WT). Conversely, TaTPR-B1 overexpression in the common wheat variety "Fielder" significantly decreased SC compared with the WT. Moreover, we developed a PCR-based marker targeting the 32-bp insertion/deletion (InDel) between the two TaTPR-B1 alleles, which could be practical and valuable in modern wheat breeding programs for diagnostic purposes. Collectively, these findings provide insight into the genetic basis of SC in common wheat and present a valuable target with a breeder-friendly diagnostic marker for gene pyramid breeding. A candidate gene within a QTL corresponds with SC, providing a valuable target gene for modern wheat breeding.

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