RNA methyltransferase BmMettl3 and BmMettl14 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development

文献类型： 外文期刊

第一作者： Li, Bing

作者： Li, Bing;Hu, Pei;Huang, Zhi-Hao;Yang, Jia-Yue;Wang, Jie;Xie, Xiu-Zhi;Wang, Yu-Han;Li, Chen-Chen;Xu, Jia-Ping;Li, Bing;Wang, Jie;Hu, Pei;Huang, Zhi-Hao;Yang, Jia-Yue;Wang, Yu-Han;Li, Chen-Chen;Xu, Jia-Ping

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关键词： Bombyx mori; cell cycle progression; diapause; embryonic development; m6A methyltransferase

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：2.2； 五年影响因子：2.1 ）

ISSN： 0739-4462

年卷期： 2023 年

页码：

收录情况： SCI

摘要： N6-methyladenosine (m6A) is a ubiquitous reversible epigenetic RNA modification that plays an important role in regulating many biological processes, especially embryonic development. However, regulation of m6A methylation during silkworm embryonic development and diapause remains to be investigated. In this study, we analyzed the phylogeny of subunits of methyltransferases BmMettl3 and BmMettl14, and detected the expression patterns of BmMettl3 and BmMettl14 in different tissues and at different developmental stages in silkworm. To investigate the function of m6A on the development of silkworm embryo, we analyzed the m6A/A ratio in diapause and diapause termination eggs. The results showed that BmMettl3 and BmMettl14 were highly expressed in gonads and eggs. Moreover, the expression of BmMettl3 and BmMettl14 and the m6A/A ratio were significantly increased in diapause termination eggs compared with diapause eggs in the early stage of silkworm embryonic development. Furthermore, in BmN cell cycle experiments, the percentage of cells in the S phase increased when lacking BmMettl3 or BmMettl14. This work contributes to understanding the role of m6A methylation during insect embryogenesis and gametogenesis. It also provides a research orientation to further analyze the role of m6A methylation in diapause initiation and termination during insect embryonic development.

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