Identification and Molecular Characterization of a 16SrII-A Phytoplasma Associated with Cucumber Phyllody in China
文献类型： 外文期刊
第一作者： Xi, Youwei
作者： Xi, Youwei;Du, Mengdan;Tang, Yafei;She, Xiaoman;Lan, Guobing;Yu, Lin;Ding, Shanwen;He, Zifu;Li, Zhenggang
作者机构：
关键词： 16SrII-A; China; cucumber; phyllody; phytoplasma
期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）
ISSN：
年卷期： 2024 年 14 卷 8 期
页码：
收录情况： SCI
摘要： Cucumber plants (Cucumis sativus) displaying symptoms of phyllody, sterility, and flower virescence were observed in fields located in the Dianbai district of Guangdong province, China. Total DNA was extracted from the symptomatic plants; this was followed by molecular detection using a set of universal phytoplasma primer pairs, R16mF2/mR1, P1/P7, and SecYF1(II)/SecYR1(II). This resulted in the PCR amplification of products corresponding to expected sizes of 1.4 kb, 1.8 kb and 1.7 kb, respectively. The 16S rDNA sequence obtained exhibited 100% similarity with the eggplant phyllody phytoplasma, the 'Cleome rutidosperma' witches' broom phytoplasma, and the 'Desmodium ovalifolium' witches' broom phytoplasma strain DeOWB, all of which belong to the 16SrII group. Phylogenetic analysis, based on the 16S rDNA gene and SecY gene sequences, confirmed the close affiliation of the detected phytoplasma isolate, tentatively designated as cucumber phyllody phytoplasma (CuPh) China isolate, with the 16SrII-A subgroup. Additionally, virtual restriction fragment length polymorphism (RFLP) analysis of the 16S rDNA sequence revealed a pattern that was identical to that of the 16SrII-A subgroup. This is the first report of cucumber phyllody phytoplasma in China.
分类号：
- 相关文献
作者其他论文 更多>>
-
Nanoscale carbon quantum dots activate antiviral immune responses and modulate the heterogeneous nuclear ribonucleoprotein interface by inducing differential alternative splicing
作者：Farooq, Tahir;He, Zifu;Tang, Yafei;She, Xiaoman;Farooq, Tahir;He, Zifu;Tang, Yafei;She, Xiaoman;Hussain, Muhammad Dilshad;Kamran, Ali;Liu, Dandan
关键词：Nanoparticles; CLCuMuV; Alternative splicing; Plant defense; Antiviral immunity; hnRNP
-
Identification and Genome Characterization of Begomovirus and Satellite Molecules Associated with Lettuce (Lactuca sativa L.) Leaf Curl Disease
作者：Tang, Yafei;Du, Mengdan;Li, Zhenggang;Yu, Lin;Lan, Guobing;Ding, Shanwen;Farooq, Tahir;He, Zifu;She, Xiaoman;Tang, Yafei;Du, Mengdan;Li, Zhenggang;Yu, Lin;Lan, Guobing;Ding, Shanwen;He, Zifu;She, Xiaoman;Du, Mengdan
关键词：lettuce leaf curl disease; pepper leaf curl Yunnan virus; tomato leaf curl China betasatellite; ageratum yellow vein alphasatellite; new geminialphasatellite
-
YiMu-QingGong san alleviates lipopolysaccharide-induced endometritis in mice via inhibiting inflammation and oxidative stress through regulating macrophage polarization
作者：Yu, Yaming;Mao, Ningning;Yu, Lin;Lin, Fangzhu;Shi, Xiaofeng;Lu, Xuanqi;Yang, Yang;Wang, Deyun;Yu, Yaming;Mao, Ningning;Yu, Lin;Lin, Fangzhu;Shi, Xiaofeng;Lu, Xuanqi;Yang, Yang;Wang, Deyun;Lu, Yu
关键词：Macrophage polarization; Inflammation; Endometritis; NF-kappa B; Fibrosis; Uterine barrier
-
Cotton leaf curl Multan virus activates autophagy in the whitefly AsiaII7, weakening its vectorial capacity for transmission
作者：Chen, Ting;Jia, Yanbo;Tang, Yafei;Chen, Jie;Qi, Guojun;Jia, Yanbo;Xu, Haiyun
关键词：
Bemisia tabaci ; cotton leaf curl Multan virus; autophagy -
Identification and genomic insights into Bacillus siamensis strains with host colonization potential and activity against tomato bacterial wilt
作者：Ding, Shanwen;Li, Ping;Tang, Yafei;He, Zifu;She, Xiaoman
关键词：
Bacillus siamensis ; bacterial wilt; antimicrobial activity; colonization; genomic analysis -
Unveiling the complete genome sequence of Paenibacillus taichungensis: genomic features and biocontrol potential
作者：Ding, Shanwen;Ma, Zijun;Tang, Yafei;Yu, Lin;He, Zifu;She, Xiaoman
关键词：
Paenibacillus taichungensis ; Antibacterial activity; Complete genome; Secondary metabolites -
Characterisation of a Betasatellite Associated With Tomato Yellow Leaf Curl Guangdong Virus and Discovery of an Unusual Modulation of Virus Infection Associated With C4 Protein
作者：Li, Zhenggang;Tang, Yafei;She, Xiaoman;Yu, Lin;Lan, Guobing;Ding, Shanwen;He, Zifu
关键词：betasatellite; C4; DNA methylation; modulation; tomato yellow leaf curl Guangdong virus; virus infection
