A dynamic regulome of shoot-apical-meristem-related homeobox transcription factors modulates plant architecture in maize

文献类型： 外文期刊

第一作者： Luo, Zi

作者： Luo, Zi;Miao, Xinxin;Zhao, Shiya;Shang, Xiaoyang;Hu, Hongyan;Yang, Fang;Li, Lin;Wu, Leiming;Zhang, Shuang;Wei, Ningning;Xue, Jiquan;Xu, Shutu;Zhang, Tifu;Li, Lin;Li, Lin;Li, Lin

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关键词： Homeobox family; Transcription factor; Binding sites; tsCUT&Tag; Plant height; Shoot architecture; Maize

期刊名称：GENOME BIOLOGY （ 影响因子：9.4； 五年影响因子：16.3 ）

ISSN： 1474-760X

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Background: The shoot apical meristem (SAM), from which all above-ground tissues of plants are derived, is critical to plant morphology and development. In maize (Zea mays), loss-of-function mutant studies have identified several SAM-related genes, most encoding homeobox transcription factors (TFs), located upstream of hierarchical networks of hundreds of genes. Results: Here, we collect 46 transcriptome and 16 translatome datasets across 62 different tissues or stages from the maize inbred line B73. We construct a dynamic regulome for 27 members of three SAM-related homeobox subfamilies (KNOX, WOX, and ZF-HD) through machine-learning models for the detection of TF targets across different tissues and stages by combining tsCUT&Tag, ATAC-seq, and expression profiling. This dynamic regulome demonstrates the distinct binding specificity and co-factors for these homeobox subfamilies, indicative of functional divergence between and within them. Furthermore, we assemble a SAM dynamic regulome, illustrating potential functional mechanisms associated with plant architecture. Lastly, we generate a wox13a mutant that provides evidence that WOX13A directly regulates Gn1 expression to modulate plant height, validating the regulome of SAM-related homeobox genes. Conclusions: The SAM-related homeobox transcription-factor regulome presents an unprecedented opportunity to dissect the molecular mechanisms governing SAM maintenance and development, thereby advancing our understanding of maize growth and shoot architecture.

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