Rapid detection of grapevine berry inner necrosis virus using a simple reverse transcription loop-mediated amplification method
文献类型： 外文期刊
第一作者： Zhang, Ying
作者： Zhang, Ying;Yuan, Qingyun;Ren, Fang;Hu, Guojun;Fan, Xudong;Dong, Yafeng
作者机构：
关键词： Grapevine; Grapevine berry inner necrosis virus (GINV); Detection; RT-LAMP
期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.8； 五年影响因子：2.8 ）
ISSN： 0885-5765
年卷期： 2024 年 132 卷
页码：
收录情况： SCI
摘要： To develop a simple method for the detection of grapevine berry inner necrosis (GINV) that does not depend on expensive instruments, a reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assay was developed. A set of primers was used in RT-LAMP to specifically detect GINV and different GINV variants belonging to groups 1, 2 and 3. The sensitivity of the RT-LAMP was about 100 times greater than that of conventional RT-PCR. Furthermore, a rapid "pin-prick" assay without RNA extraction was developed to detect GINV in RT-LAMP. The assay performed well for the detection of GINV from different parts of field grapevine plants and micropropagated grapevine plantlets, and had a higher detection rate of GINV than RT-PCR in the tested grapevine samples.
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