In Vitro Antiviral Effect of Compound Chinese Herbal Medicine and Probiotic Fermentation Effect on Siniperca chuatsi

文献类型： 外文期刊

第一作者： Tang, Jiang-Yi

作者： Tang, Jiang-Yi;Wang, Yue;Zhang, De-Feng;Wang, Qing;Shi, Cun-Bin;Wang, Ying-Ying;Wang, Ya-Jun;Tang, Jiang-Yi;Chen, Jian-Chou;Wang, Yue;Zhu, Xue-Zhi;Liu, Li-Yan

作者机构：

关键词： antiviral effect; compounded Chinese herbs; fermented herbal medicine; mandarin fish growth; probiotics

期刊名称：AQUACULTURE RESEARCH （ 影响因子：1.9； 五年影响因子：2.2 ）

ISSN： 1355-557X

年卷期： 2024 年 2024 卷

页码：

收录情况： SCI

摘要： Siniperca chuatsi is an economically important carnivorous fish. The efficient commercial culture of this fish is hindered by suboptimal nutrient utilization and the emergence of diseases, specifically viral diseases. Compound Chinese herbal medicine (CCHM) and probiotics can be used to enhance disease resistance and improve digestion. We have previously isolated Bacillus velezensis, a probiotic with a desirable effect on S. chuatsi. In this study, we aimed to evaluate the antiviral effect of a CCHM compound at the cellular level by conducting animal tests to investigate the synergistic effect of CCHM and B. velezensis GY65 in a simulated S. chuatsi culture. The animal experiments were divided into four groups: control group (G0); CCHM group (G1); B. velezensis GY65 group (G2); and Chinese herbal medicine compound probiotic group (CHCP) (G3). CCHM showed significant blocking and killing effects on the largemouth bass virus (LMBV) (p < 0.05), while the inhibiting effect was not significant. In animal tests, CCHM significantly improved S. chuatsi growth, while CHCP reduced S. chuatsi mortality. Regarding biochemical and nonspecific immune parameters, CHCP reduced serum lipid and hepatic malondialdehyde levels and increased serum lysozyme and hepatic catalase activities (p < 0.05). CCHM and B. velezensis GY65 increased the serum glutathione transaminase levels (p < 0.05). Digestive enzymes in the intestine of S. chuatsi improved in all groups, and the best result was observed in the CHCP group. Additionally, the intestinal development improved in all groups, but the best intestinal development was observed in the CCHM group. However, in the kidney, only TNF-alpha was significantly upregulated in the CHCP group (p < 0.05). In conclusion, CCHM exerted significant blocking and killing effects on LMBV. CHCP effectively protected the growth and immunity of S. chuatsi. Our findings provide preliminary data and lay the foundation for the practical application of herbal medicines and probiotics in aquaculture.

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