Phytochrome-interacting factor PIF3 integrates phytochrome B and UV-B signaling pathways to regulate gibberellin- and auxin-dependent growth in cucumber hypocotyls

文献类型： 外文期刊

第一作者： Zhao, Jianyu

作者： Zhao, Jianyu;Bo, Kailiang;Pan, Yupeng;Weng, Yiqun;Bo, Kailiang;Yu, Daoliang;Gu, Xingfang;Pan, Yupeng;Li, Yuhong;Li, Chuang;Wang, Zhongyi;Zhang, Xiaolan;Chang, Jiang;Wu, Shuang;Weng, Yiqun

作者机构：

关键词： Cucumber; Cucumis sativus; hypocotyl growth; light signaling crosstalk; photomorphogenesis; phytohormone signaling; UV-B responses

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年 74 卷 15 期

页码：

收录情况： SCI

摘要： In Arabidopsis, the photoreceptors phytochrome B (PhyB) and UV-B resistance 8 (UVR8) mediate light responses that play a major role in regulating photomorphogenic hypocotyl growth, but how they crosstalk to coordinate this process is not well understood. Here we report map-based cloning and functional characterization of an ultraviolet (UV)-B-insensitive, long-hypocotyl mutant, lh1, and a wild-type-like mutant, lh2, in cucumber (Cucumis sativus), which show defective CsPhyB and GA oxidase2 (CsGA20ox-2), a key gibberellic acid (GA) biosynthesis enzyme, respectively. The lh2 mutation was epistatic to lh1 and partly suppressed the long-hypocotyl phenotype in the lh1lh2 double mutant. We identified phytochrome interacting factor (PIF) CsPIF3 as playing a critical role in integrating the red/far-red and UV-B light responses for hypocotyl growth. We show that two modules, CsPhyB-CsPIF3-CsGA20ox-2-DELLA and CsPIF3-auxin response factor 18 (CsARF18), mediate CsPhyB-regulated hypocotyl elongation through GA and auxin pathways, respectively, in which CsPIF3 binds to the G/E-box motifs in the promoters of CsGA20ox-2 and CsARF18 to regulate their expression. We also identified a new physical interaction between CsPIF3 and CsUVR8 mediating CsPhyB-dependent, UV-B-induced hypocotyl growth inhibition. Our work suggests that hypocotyl growth in cucumber involves a complex interplay of multiple photoreceptor- and phytohormone-mediated signaling pathways that show both conservation with and divergence from those in Arabidopsis. Cucumber phytochrome-interacting factor PIF3 physically interacts with both the photoreceptors phytochrome B and UV-B resistance 8, thereby connecting the red/far-red and ultraviolet-B light response pathways for hypocotyl growth.

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