The comparative transcriptome analysis of two green super rice genotypes with varying tolerance to salt stress

文献类型： 外文期刊

第一作者： Zahra, Nageen

作者： Zahra, Nageen;Uzair, Muhammad;Zaid, Imdad Ullah;Inam, Safeena;Naeem, Muhammad Kashif;Farooq, Umer;Rehman, Nazia;Khan, Muhammad Ramzan;Attia, Kotb A.;Fiaz, Sajid;Abdallah, Rizk M.;Ali, Ghulam Muhammad;Xu, Jianlong;Li, Zhikang

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关键词： Rice; Salinity; RNA-seq; Differentially expressed genes; Molecular pathways

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2024 年 51 卷 1 期

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收录情况： SCI

摘要： BackgroundSalinity is one of the main abiotic factors that restrict plant growth, physiology, and crop productivity is salt stress. About 33% of the total irrigated land suffers from severe salinity because of intensive underground water extraction and irrigation with brackish water. Thus, it is important to understand the genetic mechanism and identify the novel genes involved in salt tolerance for the development of climate-resilient rice cultivars.Methods and resultsIn this study, two rice genotypes with varying tolerance to salt stress were used to investigate the differential expressed genes and molecular pathways to adapt under saline soil by comparative RNA sequencing at 42 days of the seedling stage. Salt-susceptible (S3) and -tolerant (S13) genotypes revealed 3982 and 3463 differentially expressed genes in S3 and S13 genotypes. The up-regulated genes in both genotypes were substantially enriched in different metabolic processes and binding activities. Biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, and plant signal transduction mechanisms were highly enriched. Salt-susceptible and -tolerant genotypes shared the same salt adaptability mechanism with no significant quantitative differences at the transcriptome level. Moreover, bHLH, ERF, NAC, WRKY, and MYB transcription factors were substantially up-regulated under salt stress. 391 out of 1806 identified novel genes involved in signal transduction mechanisms. Expression profiling of six novel genes further validated the findings from RNA-seq data.ConclusionThese findings suggest that the differentially expressed genes and molecular mechanisms involved in salt stress adaptation are conserved in both salt-susceptible and salt-tolerant rice genotypes. Further molecular characterization of novel genes will help to understand the genetic mechanism underlying salt tolerance in rice.

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