alpha-Glucosidase inhibitors from brown rice bound phenolics extracts (BRBPE): Identification and mechanism

文献类型： 外文期刊

第一作者： Ye, Caiyan

作者： Ye, Caiyan;Zhang, Ruifen;Chi, Jianwei;Huang, Fei;Dong, Lihong;Zhang, Mingwei;Jia, Xuchao;Dong, Limei

作者机构：

关键词： Brown rice; Bound phenolics; alpha-Glucosidase inhibitory activity; Ultrafiltration; Inhibitory kinetics; Molecular simulation

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 372 卷

页码：

收录情况： SCI

摘要： Brown rice bound phenolics extracts (BRBPE) have been reported to possess alpha-glucosidase inhibitory effects, the specific enzyme inhibitors involved in this process were unknown. Here, alpha-glucosidase inhibitors in BRBPE were screened using bioaffinity ultrafiltration methods, and seven phenolic compounds - three monomers (p-coumaric acid, ferulic acid and methyl ferulate), three dimers (8-5', 5-5' and 8-O-4' diferulic acid) and a trimer (5-5'/8-O4 '' dehydrotriferulic acid) were identified as exact inhibitors, among which 5-5' /8-O-4 '' dehydrotriferulic acid and 5-5' diferulic acid exhibited the best inhibitory activity. Enzyme kinetic analysis suggested that the inhibitory mechanism of these seven inhibitors including competitive, noncompetitive, uncompetitive and mixed manner. Molecular docking analysis revealed that the seven inhibitors bind with alpha-glucosidase mainly by hydrogen bonding interaction, hydrophobic force and ionic bond. Molecular dynamics simulation further explored the structure and molecular property of phenolic-glucosidase complex. This work provided a deep insight into brown rice bound phenolics acting as potent a-glucosidase inhibitors.

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