Association Between Susceptibility of Thrips palmi to Spinetoram and Frequency of G275E Mutation Provides Basis for Molecular Quantification of Field-Evolved Resistance

文献类型： 外文期刊

第一作者： Shi, Pan

作者： Shi, Pan;Guo, Shao-Kun;Gao, Yong-Fu;Chen, Jin-Cui;Gong, Ya-Jun;Tang, Meng-Qing;Cao, Li-Jun;Wei, Shu-Jun;Shi, Pan;Tang, Meng-Qing;Li, Hu;Wei, Shu-Jun;Shi, Pan;Tang, Meng-Qing;Li, Hu;Wei, Shu-Jun;Hoffmann, Ary Anthony

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关键词： Thrips palmi; spinosyns; nicotinic acetylcholine receptors; mutation; molecular diagnostics

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN： 0022-0493

年卷期： 2021 年 114 卷 1 期

页码：

收录情况： SCI

摘要： Putative mechanisms underlying spinosyn resistance have been identified in controlled studies on many species; however, mechanisms underlying field-evolved resistance and the development of a molecular diagnostic method for monitoring field resistance have lagged behind. Here, we examined levels of resistance of melon thrips, Thrips palmi Karny (Thysanoptera:Thripidae), to spinetoram as well as target site mutations in field populations across China to identify potential mechanisms and useful molecular markers for diagnostic and quantifying purposes. In resistant populations, we identified the G275E mutation, which has previously been linked to spinosyns resistance, and F314V mutation, both located in the alpha 6 subunit of the nicotinic acetylcholine receptor. There was a strong correlation between levels of spinetoram resistance and allele frequency of G275E mutation in field-collected populations (r(2) = 0.84) and those reared under laboratory conditions for two to five generations (r(2) = 0.91). LC50 ranged from 0.12 to 0.66 mg/liter in populations without G275E mutation, whereas it ranged from 33.12 to 39.91 mg/liter in most populations with a G275E mutation frequency more than 90%. Our results indicate that the field-evolved resistance of T. palmi to spinetoram in China is mainly conferred by the G275E mutation. The frequency of the G275E mutation provides a useful diagnostic for quantifying resistance levels in field populations of T. palmi.

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