Wheat TaSnRK2.10 phosphorylates TaERD15 and TaENO1 and confers drought tolerance when overexpressed in rice

文献类型： 外文期刊

第一作者： Zhang, Yanfei

作者： Zhang, Yanfei;Li, Yuying;Wang, Chenyang;Zhang, Yanfei;Wang, Jingyi;Li, Yuying;Zhang, Zihui;Yang, Lili;Wang, Min;Zhang, Yining;Zhang, Jie;Li, Chaonan;Li, Long;Jing, Ruilian;Mao, Xinguo;Zhang, Zihui;Zhang, Yining;Mao, Xinguo;Zhang, Jie;Reynolds, Matthew P.

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2023 年 191 卷 2 期

页码：

收录情况： SCI

摘要： Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) acts as a signaling hub in the response to drought stress, but whether SnRK2 helps plants cope with water deficit via other mechanisms is largely unknown. Here, we cloned and characterized TaSnRK2.10, which was induced by multiple abiotic stresses and phytohormones. Ectopic expression of TaSnRK2.10 in rice (Oryza sativa) conferred drought tolerance, manifested by multiple improved physiological indices, including increased water content, cell membrane stability, and survival rates, as well as decreased water loss and accumulation of H2O2 and malonaldehyde. TaSnRK2.10 interacted with and phosphorylated early responsive to dehydration 15 (TaERD15) and enolase 1 (TaENO1) in vivo and in vitro. TaERD15 phosphorylated by TaSnRK2.10 was prone to degradation by the 26S proteasome, thereby mitigating its negative effects on drought tolerance. Phosphorylation of TaENO1 by TaSnRK2.10 may account for the substantially increased levels of phosphoenolpyruvate (PEP), a key metabolite of primary and secondary metabolism, in TaSnRK2.10-overexpressing rice, thereby enhancing its viability under drought stress. Our results demonstrate that TaSnRK2.10 not only regulated stomatal aperture and the expression of drought-responsive genes, but also enhanced PEP supply and promoted the degradation of TaERD15, all of which enhanced drought tolerance. Wheat sucrose non-fermenting 1-related protein kinase 2.10 confers drought tolerance in overexpressed rice and phosphorylates the early responsive to dehydration 15 and enolase 1.

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