Variation in the affinity of three representative avian adenoviruses for the cellular coxsackievirus and adenovirus receptor

文献类型： 外文期刊

第一作者： Song, Yapeng

作者： Song, Yapeng;Tao, Mingyue;Liu, Lin;Wang, Yang;Zhao, Zhenchao;Huang, Zongmei;Gao, Wenming;Li, Xinsheng;Wei, Qiang

作者机构：

关键词： Avian adenovirus; fibre; crystal structure; coxsackievirus-adenovirus receptor; affinity variation

期刊名称：VETERINARY RESEARCH （ 影响因子：4.4； 五年影响因子：4.3 ）

ISSN： 0928-4249

年卷期： 2024 年 55 卷 1 期

页码：

收录情况： SCI

摘要： According to previous studies, three representative avian adenoviral strains utilize coxsackievirus-adenovirus receptor (CAR) as a receptor and seem to exhibit diverse binding affinities and modes. Thus, further revealing the exact molecular mechanism underlying the interaction between different FAdVs and the attachment receptor CAR is necessary. In this study, we successfully solved the crystal structure of the FAdV-4 fiber1 knob at 1.6 angstrom resolution. The interaction between the fibre knob and different domains of CAR was verified by confocal microscopy, coimmunoprecipitation and surface plasmon resonance (SPR) analysis. The fibre knobs of the three representative fowl adenoviruses specifically recognized CAR domain 1 (D1), but the recognition of CAR domain 2 (D2) by chicken embryo lethal orphan (CELO) strains was weak. These results provide insights into the differences in adenovirus-host cell interactions and have important implications for the exploration of viral invasion mechanisms.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序