Transplastomic RNAi potato plants targeting potato ladybird: No adverse effects on non-target pests or their natural enemies

文献类型： 外文期刊

第一作者： Liao, Jianghua

作者： Liao, Jianghua;He, Haofeng;Liu, Xia;Chang, Ling;Zhang, Jiang;Liao, Jianghua;He, Haofeng;Liu, Xia;Zhang, Jiang

作者机构：

关键词： RNA interference; double-stranded RNA; potato; Henosepilachna vigintioctopunctata; non-target insects

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2025 年 45 卷 3 期

页码：

收录情况： SCI

摘要： RNA interference (RNAi) technology has emerged promising and eco-friendly method for crop protection against pests. Our previous work has demonstrated the effectiveness of plastid-expressed double-stranded RNAs (dsRNAs) targeting insect beta-Actin (dsActin) and Snap (dsSnap) genes in controlling Henosepilachna vigintioctopunctata (Fabricius) (Coleoptera, Coccinellidae). However, the potential impacts of these RNAi transplastomic plants on non-target organisms remains ambiguous. In this study, we investigated the effects of transplastomic plants expressing dsActin or dsSnap on two potato pests, Myzus persicae (Sulzer) (Homoptera, Aphididae) and Spodoptera litura (Fabricius) (Lepidoptera, Noctuidae), along with two natural enemies of potato pests, Arma chinensis (Fallou) (Hemiptera, Pentatomidae) and Harmonia axyridis (Pallas) (Coleoptera, Coccinellidae). Despite HvActin sharing over 85% gene homology and HvSnap sharing over 60% with these non-target organisms, our findings revealed that neither in vitro-synthesized nor plastid-expressed dsActin or dsSnap caused any transcriptional or organismal impacts on these four non-target species. In sum, our study verifies the safety of transplastomic plants towards non-targe organisms, thus paving the way for the widespread adoption of transplastomic RNAi crops.

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