DUO1 Activated Zinc Finger (AtDAZ) protein role in the generative cell body morphogenesis

文献类型： 外文期刊

第一作者： Rauf, Abdur

作者： Rauf, Abdur;Wang, Anbang;Li, Yujia;Lian, Zhihao;Wei, Shouxing;Li, Jingyang;Rauf, Abdur;Rauf, Abdur;Khan, Qayash;Jabbar, Kashmala;Jan, Farooq;Khan, Ikramullah;Bibi, Mamoona;Abidullah, Syed;Rauf, Abdur;Li, Jingyang

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关键词： Generative cell; T-DNA; MYB-TF; DUO1 target genes; DUO1-DAZ1; Morphogenesis

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：3.8； 五年影响因子：4.6 ）

ISSN： 0167-4412

年卷期： 2025 年 115 卷 1 期

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收录情况： SCI

摘要： Arabidopsis MYB transcription factor, AtDUO1 regulates generative cell body (GC) morphogenesis from round to semi and fully elongated forms before pollen mitosis-II (PM II). It was hypothesised that DUO1 might regulate morphogenesis through any of its direct target genes or components of the DUO1-DAZ1 network. The developmental analysis of plants harbouring T-DNA insertions in some DUO1 target genes using light and fluorescence microscopy revealed abnormal GC morphogenesis only in daz1 and daz2, but gcs1, trm16, mapkkk10, mapkkk20, tet11, and tip1 all undergo normal elongation indicating that these target genes have no important roles in morphogenesis or may be redundant. The important regulatory role of DUO1 was confirmed through the observed incomplete rescue of morphogenesis of mutant duo1-1 GCs by DAZ1 and independently by a C-terminally deleted version of DUO1 (DUO1 triangle C3) lacking activation sequences. The evidence supports the important role of DAZ1 in GC shape partial morphogenesis. The C-terminus of DUO1 may regulate some target genes that affect GC body elongation. Furthermore, an intact DUO1 is shown to be indispensable for GC shape and nucleus elongation and subsequently for timely division and sperm cell morphogenesis. The development of the GC cytoplasmic projection is regulated independently of DUO1, and all its target genes were able to form it.

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