RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites

文献类型： 外文期刊

第一作者： Huang, Juan

作者： Huang, Juan;Du, Juan;Liu, Yan;Lu, Lu;Xu, Yanzhuo;Shi, Jianfei;Liu, Qing;Li, Qi;Liu, Yang;Chen, Yaqiu;Du, Meng;Zhao, Yiming;Chen, Jinfeng;Cui, Feng;Zhang, Xiaoming;Huang, Juan;Du, Juan;Xu, Yanzhuo;Shi, Jianfei;Liu, Qing;Liu, Yang;Zhao, Yiming;Cui, Feng;Zhang, Xiaoming;Liu, Yan;Huo, Liangxiao;Wang, Weiran;Ding, Chenxi;Wei, Liya;Wu, Jianguo;Yuan, Yao-Wu;Li, Ruixi;Zhang, Xiaoming

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：15.7； 五年影响因子：17.2 ）

ISSN：

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCI

摘要： While RNA silencing is crucial for plant resistance against viruses, the cellular connections between RNA silencing and antiviral responses in plants remain poorly understood. In this study, we aim to investigate this relationship by examining the subcellular localization of small RNA loading and viral replication in Arabidopsis. Our findings reveal that Argonaute 2 (AGO2), a key component of RNA silencing, loads small RNAs at the endoplasmic reticulum (ER)-chloroplast membrane contact sites (MCSs). We identify a chloroplast-localized protein, RNA helicase 3 (RH3), which interacts with AGO2 and facilitates the loading of small RNAs into AGO2 at these MCSs. Furthermore, we discover that MCSs serve as replication sites for certain plant viruses. RH3 also promotes the loading of viral-derived small RNAs into AGO2, thereby enhancing plant antiviral resistance. Overall, our study sheds light on the roles of RH3 in RNA silencing and plant antiviral defenses, providing valuable insights into the cytobiological connections between RNA silencing, viral replication, and antiviral immunity.

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