Effects of neuropeptide F signaling on feeding, growth and development of Plutella xylostella (L.) larvae

文献类型： 外文期刊

第一作者： Shen, Cheng

作者： Shen, Cheng;Wu, Jiayi;Huang, Ziyan;He, Meng;Chen, Wei;Ilyas, Naila;Zhang, Xiao;Chen, Chengyu;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Shen, Cheng;Huang, Ziyan;Liu, Yuan;Liu, Xianjin;Wu, Jiayi;He, Meng;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Wang, Yun

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关键词： Plutella xylostella; Neuropeptide F signaling; Feeding regulation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 293 卷

页码：

收录情况： SCI

摘要： The neuropeptide F (NPF) signaling, comprising NPF and neuropeptide F receptor (NPFR), role in regulating insect behaviors and physiological processes. We cloned the genes encoding NPF and NPFR from Plutella xylostella, a notorious pest of cruciferous crops. Notably, the NPF gene produced two splicing variants, Px-NPF1 and Px-NPF2, with distinct expression patterns. Conserved C-terminal RPRFamide motif and seven transmembrane alpha-helics were observed in Px-NPF and in Px-NPFR, respectively. Px-NPF and Px-NPFR are widely expressed across all larval instars and are predominantly localized in the brain and midgut. The transcriptional levels of Px-NPF1 and Px-NPFR increased and reached maximum at 8 h after food deprived. RNA interference targeting Px-NPF and Px-NPFR resulted in smaller body size, delayed larval growth and alterations in energy metabolism. Specific binding assays revealed stronger binding affinity of Px-NPF1 with Px-NPFR than Px-NPF2. Three-dimensional models of the Px-NPF-NPFR complexes via AlphaFold 3 highlighted the critical roles of two highly conserved residues across insects, Tyr46 and His196, in interaction. Our findings underscore the potential of targeting NPF-NPFR signaling for P. xylostella controlling and contributing to developing novel pest management strategies.

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