Development of real-time recombinase polymerase amplification (RPA) and RPA combined with lateral flow dipstick (LFD) assays for the rapid and sensitive detection of cyprinid herpesvirus 3

文献类型： 外文期刊

第一作者： Li, Yingying

作者： Li, Yingying;Li, Ruifan;Mo, Xubing;Wang, Yingying;Yin, Jiyuan;Ren, Yan;Pan, Houjun;Shi, Cunbin;Zhang, Defeng;Wang, Qing;Bergmann, Sven M.;Zhang, Defeng;Wang, Qing

作者机构：

关键词： cyprinid herpesvirus 3(CyHV-3); real-time RPA; RPA-LFD; sera

期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.5； 五年影响因子：2.6 ）

ISSN： 0140-7775

年卷期： 2024 年

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收录情况： SCI

摘要： In this issue, we established rapid, cost-effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time RPA for cyprinid herpesvirus 3(CyHV-3), and evaluated their sensitivity, specificity, and applicability, the real-time RPA method could achieve sensitive diagnosis of CyHV-3 within 1.3 copies per reaction, respectively. The real-time RPA method is 10-fold more sensitive than RPA-LFD method. The exact number of CyHV-3 can be calculated in each sample by real-time RPA. The sera from koi also can be tested in these methods. In addition, no cross-reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV-1), cyprinid herpesvirus 2(CyHV-2), type I grass carp reovirus (GCRV-I), type II GCRV (GCRV-II), type III GCRV (GCRV-III), and Aeromonas hydrophila.

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