Evaluation of the Control Efficacy of Bt Maize Expressing Cry1Ab and Vip3Aa Proteins Against Agrotis ypsilon (Rottemberg)

文献类型： 外文期刊

第一作者： Wang, Wenhui

作者： Wang, Wenhui;He, Yuting;Yu, Huan;Yang, Xianming;Wu, Kongming;Wang, Wenhui;He, Yuting

作者机构：

关键词： Agrotis ypsilon; Bt maize; susceptibility; preference

期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）

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年卷期： 2025 年 16 卷 2 期

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收录情况： SCI

摘要： Agrotis ypsilon (Rottemberg) is a worldwide major pest of maize seedlings. Currently, Bt maize is the main means of controlling lepidopteran pests, but its control efficacy against A. ypsilon is unclear. We studied the toxicity of Bt proteins expressed by DBN9936 (expressing Cry1Ab), DBN9501 (expressing Vip3Aa), and DBN3601T (expressing Cry1Ab and Vip3Aa) against A. ypsilon. The bioassay showed that Vip3Aa has the most toxicity and that DBN3601T maize can effectively control the damage of the pest to maize plants. The results of behavioral tests indicated that the insect larvae exhibited antifeedant behavior toward Bt maize, while the moths demonstrated a preference for laying eggs on undamaged or minimally damaged Bt maize plants. This study provided a theoretical basis for building an integrated pest management system for A. ypsilon based on Bt maize planting. Bt maize is the main means to control many lepidopteran pests in the world, but its control efficacy against Agrotis ypsilon (Rottemberg), an important insect pest of maize seedlings, remains unclear until now. The interaction between the insect and Bt transgenic maize events (DBN9936 (expressing Cry1Ab), DBN9501 (expressing Vip3Aa), and DBN3601T (expressing Cry1Ab and Vip3Aa)) was investigated using bioassay and insect behavioral tests. The results show that the Cry1Ab contents in different tissues of DBN9936 were 47.78-82.60 mu gg(-1), and the Vip3Aa contents in DBN9501 were 15.29-27.78 mu gg(-1). The contents of Cry1Ab and Vip3Aa in DBN3601T were 32.08-79.08 and 10.16-17.52 mu gg(-1), respectively. There was no significant difference in total Bt protein content between the leaves and stems; however, that the content in both was significantly higher than that in the roots. The larvae were most sensitive to the Vip3Aa protein, and the corrected mortalities of larvae feeding on DBN9501 and DBN3601T were greater than 89.65% at the seedling stage, significantly higher than those feeding on DBN9936 (16.46-76.13%). The corrected mortalities of the third to the fifth instar larvae feeding on Bt maize root were as follows: DBN3601T (54.00-96.60%) > DBN9501 (24.67-70.88%) > DBN9936 (6.67-53.31%). The results of behavioral tests for Bt/non-Bt maize plant selection indicated that the larvae mainly fed on non-Bt maize while showing antifeedant behavior toward Bt maize, and the moth preferred to lay eggs on undamaged or slightly damaged Bt maize. It is concluded that DBN3601T maize has a strong control efficacy for A. ypsilon, which can play an important role in building an integrated pest management strategy for the insect.

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