Canine distemper virus N protein induces autophagy to facilitate viral replication

文献类型： 外文期刊

第一作者： Chen, Fei

作者： Chen, Fei;Zhang, Zhixiong;Bai, Ling;Zhao, Shuaiyang;Wu, Yongshu;Guo, Zijing;Zhang, Rui;Zhang, Zhidong;Li, Yanmin;Hu, Bo

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关键词： Canine distemper virus; Autophagy; Replication; Nucleocapsid protein

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.9 ）

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年卷期： 2023 年 19 卷 1 期

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收录情况： SCI

摘要： BackgroundCanine distemper virus (CDV) is one of the most contagious and lethal viruses known to the Canidae, with a very broad and expanding host range. Autophagy serves as a fundamental stabilizing response against pathogens, but some viruses have been able to evade or exploit it for their replication. However, the effect of autophagy mechanisms on CDV infection is still unclear.ResultsIn the present study, autophagy was induced in CDV-infected Vero cells as demonstrated by elevated LC3-II levels and aggregation of green fluorescent protein (GFP)-LC3 spots. Furthermore, CDV promoted the complete autophagic process, which could be determined by the degradation of p62, co-localization of LC3 with lysosomes, GFP degradation, and accumulation of LC3-II and p62 due to the lysosomal protease inhibitor E64d. In addition, the use of Rapamycin to promote autophagy promoted CDV replication, and the inhibition of autophagy by Wortmannin, Chloroquine and siRNA-ATG5 inhibited CDV replication, revealing that CDV-induced autophagy facilitated virus replication. We also found that UV-inactivated CDV still induced autophagy, and that nucleocapsid (N) protein was able to induce complete autophagy in an mTOR-dependent manner.ConclusionsThis study for the first time revealed that CDV N protein induced complete autophagy to facilitate viral replication.

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