Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae

文献类型： 外文期刊

第一作者： Li ZhiYong

作者： Li ZhiYong;Yi YongZhu;Yin XiangPing;Zhang ZhiFang;Liu JiXing

作者机构：

关键词： foot-and-mouth disease virus;open reading frame;silkworm-baculovirus expression system;immunogenicity;subunit vaccine

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： The intact open reading frame (ORF) of foot-and-mouth disease virus (FMDV) Asia I/XJ strain was amplified by RT-PCR and inserted into the transfer vector pVL1393 to generate plasmid pVL-ORF. Bm-N cells were transfected with pVL-ORF and linearized Sm-BacPAK6 DNA, and the recombinant silkworm baculovirus Sm-ORF containing the full ORF of FMDV was obtained. The results of indirect im-munofluorescence assay (IFA) showed that Bm-ORF could be expressed efficiently in Bm-N cell. After inoculating the early5th instar larvae of silkworm, the polyprotein of FMDV could be detected by sandwich ELISA and empty capsid-like particles could be observed under the electron microscope. Expression products from silkworm were used as the antigen to immunize the cattle.The specific antibody was induced in all vaccinated animals. The immunized cattle were challenged with the virulent FMDV Asia I/XJ strain, two of the four cattle were completely protected and clinical symptoms were alleviated and delayed in the others.The results suggest that this strategy might be used to develop the new subunit FMDV vaccine.

分类号： N1

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