A novel in-situ quantitative profiling approach for visualizing changes in lignin and cellulose by stained micrographs

文献类型： 外文期刊

第一作者： Liao, Keke

作者： Liao, Keke;Han, Lujia;Yang, Zengling;Huang, Yuanping;Lyu, Qian;Shi, Zhuolin;Shi, Suan;Du, Shurong

作者机构：

关键词： Lignin; Cellulose; In-situ simultaneous quantification; Crop stalk; Safranin O-fast green staining

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：10.723； 五年影响因子：9.964 ）

ISSN： 0144-8617

年卷期： 2022 年 297 卷

页码：

收录情况： SCI

摘要： There is a strong need for low-cost lignocellulosic composition simultaneous localization methodologies to benefit deeper understandings of crop stalk morphology. This study developed a robust quantitative safranin O-fast green staining-based optical microscopy imaging methodology for in-situ simultaneously generating digital profiles of lignin and cellulose in stalk tissues. Foreground extraction and dye residue removal of stained images were adapted. The ratios of normalized red (R), green (G), and blue (B) channel signal intensity, R/B and G/B, were defined as quantitative indicators of lignin and cellulose, respectively. The method was validated on model rice with known bioinformatics, and the results were consistent with those of fluorescence microscopy and immunogold labeling methods. The high-definition spatial in-situ simultaneous profiles of lignin and cellulose in alkali-treated maize stalk tissues and their variations were visualized. This low-cost, cell-scale method is ex-pected to contribute to new discoveries in many areas of biomass refining and plant science.

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