The antioxidant protein glutaredoxin 1 is essential for oxidative stress response and pathogenicity of Toxoplasma gondii

文献类型： 外文期刊

第一作者： Li, Ting-Ting

作者： Li, Ting-Ting;Zhao, Dan-Yu;Liang, Qin-Li;Wang, Meng;Sun, Li-Xiu;Zhang, Zhi-Wei;Wang, Jin-Lei;Li, Ting-Ting;Wang, Meng;Wang, Jin-Lei;Elsheikha, Hany M.;Chen, Xiao-Qing;Zhu, Xing-Quan;Zhu, Xing-Quan;Wang, Jin-Lei;Zhu, Xing-Quan

作者机构：

关键词： biological function; glutaredoxins (Grxs); oxidative stress; Toxoplasma gondii; virulence

期刊名称：FASEB JOURNAL （ 影响因子：4.8； 五年影响因子：5.2 ）

ISSN： 0892-6638

年卷期： 2023 年 37 卷 6 期

页码：

收录情况： SCI

摘要： Glutaredoxins (Grxs) are ubiquitous antioxidant proteins involved in many molecular processes to protect cells against oxidative damage. Here, we study the roles of Grxs in the pathogenicity of Toxoplasma gondii. We show that Grxs are localized in the mitochondria (Grx1), cytoplasm (Grx2), and apicoplast (Grx3, Grx4), while Grx5 had an undetectable level of expression. We generated ?grx1-5 mutants of T. gondii type I RH and type II Pru strains using CRISPR-Cas9 system. No significant differences in the infectivity were detected between four ?grx (grx2-grx5) strains and their respective wild-type (WT) strains in vitro or in vivo. Additionally, no differences were detected in the production of reactive oxygen species, total antioxidant capacity, superoxide dismutase activity, and sensitivity to external oxidative stimuli. Interestingly, RH?grx1 or Pru?grx1 exhibited significant differences in all the investigated aspects compared to the other grx2-grx5 mutant and WT strains. Transcriptome analysis suggests that deletion of grx1 altered the expression of genes involved in transport and metabolic pathways, signal transduction, translation, and obsolete oxidation-reduction process. The data support the conclusion that grx1 supports T. gondii resistance to oxidative killing and is essential for the parasite growth in cultured cells and pathogenicity in mice and that the active site CGFS motif was necessary for Grx1 activity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序