GhTKPR1_8 functions to inhibit anther dehiscence and reduce pollen viability in cotton

文献类型： 外文期刊

第一作者： Chen, Lingling

作者： Chen, Lingling;Hao, Juxin;Qiao, Kaikai;Wang, Ningna;Pu, Xiaoyan;Fan, Shuli;Ma, Qifeng;Ma, Lina;Wang, Jin;Wang, Zhe;Fan, Shuli

作者机构：

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：6.4； 五年影响因子：5.9 ）

ISSN： 0031-9317

年卷期： 2024 年 176 卷 3 期

页码：

收录情况： SCI

摘要： Sporopollenin, as the main component of the pollen exine, is a highly resistant polymer that provides structural integrity under unfavourable environmental conditions. Tetraketone alpha-pyrone reductase 1 (TKPR1) is essential for sporopollenin formation, catalyzing the reduction of tetraketone carbonyl to hydroxylated alpha-pyrone. The functional role of TKPR1 in male sterility has been reported in flowering plants such as maize, rice, and Arabidopsis. However, the molecular cloning and functional characterization of TKPR1 in cotton remain unaddressed. In this study, we identified 68 TKPR1s from four cotton species, categorized into three clades. Transcriptomics and RT-qPCR demonstrated that GhTKPR1_8 exhibited typical expression patterns in the tetrad stage of the anther. GhTKPR1_8 was localized to the endoplasmic reticulum. Moreover, ABORTED MICROSPORES (GhAMS) transcriptionally activated GhTKPR1_8 as indicated by luciferase complementation tests. GhTKPR1_8-knockdown inhibited anther dehiscence and reduced pollen viability in cotton. Additionally, overexpression of GhTKPR1_8 in the attkpr1 mutant restored its male sterile phenotype. This study offers novel insights into the investigation of TKPR1 in cotton while providing genetic resources for studying male sterility.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序